摘要
目的:从基因水平上研究用6MV X射线照射治疗后,海马神经细胞基因表达的变化。方法:用分割剂量为2 Gy/次,50 Gy/25次6MV X射线对SD成年大鼠进行全脑照射后,以穿梭箱法分别测试其学习和记忆能力。活杀大鼠,取出海马,分别提取假照射组和照射组大鼠海马mRNA,经逆转录为cDNA后用Cy3、Cy5两种物质荧光标记,获得两组动物来源的cDNA探针,与2张基因表达谱芯片进行杂交,结果由扫描仪扫描并用软件进行统计分析。结果:①X射线导致SD成年大鼠学习和记忆功能显著降低。②与假照射组相比,有43条基因发生明显变化,其中28条基因表达水平明显升高,15条明显下降,其他基因无显著变化。③在表达异常的基因中,有17条是已有报道的功能基因,其余则为新筛选出的基因种类。结论:电离辐射对学习和记忆功能的影响是一个较为复杂的过程,其中涉及到众多的信号转导分子,并与线粒体功能异常有关。
Objective： To observe differentially expressed genes in rat hippocampus irradiated by 6 MV X-rays during radiotherapy. Methods：After whole brain irradiation of 50 Gy in 25 fractions with 2 Gy per fraction, the capability of learning and memory was investigated in a shuttle box model, then rats were killed and the hippocampus were taken, mRNA from sham irradiation group and irradiation group were reversely transcribed to cDNA with incorporation of fluorescence dUTP（ Cy5 and Cy3 dUTP） to prepare for hybridization probes. The mixed probes were hybridized to the cDNA microarray. Axon Genechix 4000 BandGenePix Pro3.0 software was used to scan and analyze the fluorescent signals. Results：① The active avoidance response of irradiated rats were lowered significantly. ②Compared with the sham group, significant changes took place in 43 genes, among which 28 genes were upregulated and 15 down regulated, other genes did not change significantly. （~）Among the 43 malexpressed genes, 16 were reported previously, others were newly found genes. Conclusion： The influence of ionizing radiation on learning and memory was a complex course, including many transduction factors and might be related to mitochondrial dysfunction.
出处
《医学研究生学报》
CAS
2007年第5期502-505,共4页
Journal of Medical Postgraduates
关键词
X射线
基因芯片
学习和记忆
海马
X-ray
DNA microarray
Learning and memory
Hippocampus
