摘要
目的探讨p53基因家族新成员截短型p63(△Np63)在食管鳞癌组织中的表达及其意义。方法采用RT-PCR法检测50例食管鳞癌及50例正常食管组织中△Np63 mRNA的表达情况,分析其与食管鳞癌患者各临床病理指标的关系。结果△Np63 mRNA在食管鳞癌组织中的阳性表达率(74.0%)及表达水平(1.036±0.157)均显著高于正常食管组织(40.0%,0.954±0.110),并且其在食管鳞癌组织中的阳性表达率及表达水平与分化程度和淋巴结转移密切相关(P<0.05)。结论在食管鳞癌组织中存在△Np63的过表达,并且这种表达可能与食管鳞癌的浸润、转移有关。
Objective To investigate the expression and significance of delta p63 （ ANp63 ）, a novel member of p53 gene family,in esophageal squamous cell carcinoma. Methods The expression of A Np63 mRNA in 50 cases of esophageal careinoma and 50 eases of normal esophageal mucoma were detected by reverse transcription-polymerase chain reaction （ RT-PCR）. And analyzed the relation among clinical patho-index of esophageal squamous cell carcinoma. Results The positive expression rate（74.0% ） and relative content（ 1. 036 ± 0. 157）of △ Np63 mRNA in esophageal squamous cell carcinoma were significantly higher than in normal esophageal mucoma （40.0%, 0. 954± 0. 110 ）. The positive expression rate and relative content of △Np63 mRNA in esophageal squamous cell carcinoma were correlated with degrees of differentiation and lymph node metastasis （ P 〈 0.05 ）. Conclusion Over expression of △ Np63 gene may be relation with invasion and lymph node metastasis in esophageal squamous cell carcinoma.
出处
《新乡医学院学报》
CAS
2007年第3期242-244,共3页
Journal of Xinxiang Medical University
关键词
食管鳞癌
△NP63
逆转录多聚酶链反应
esophageal squamous cell careinoma △ Np63 reverse transcription- polymerase chain reaction
