摘要
以冬凤兰[Cymbidium dayanum Rchb.],竹叶兰[Arundian graminifolia(D.Don)Hochr.],碧玉兰[C.lowia-num(Rchb.f.)Rchb.],西藏虎头兰(C.tracyanumL.)组培苗为材料,进行60Coγ射线不同剂量处理,统计各处理植株死亡率、成活率及成活植株增殖率,并观察当代表型变异率和进行回归模型分析,初步结果表明:冬凤兰、竹叶兰、碧玉兰、西藏虎头兰组培苗的半致死剂量分别为:20.72 Gy,26.31 Gy,29.88 Gy,41.04 Gy,出现了植株矮化、变粗、叶变宽、叶尖分叉、叶上有淡绿斑,叶扭曲等较明显的表型变化。
Treated vitro plantlets of Cymbidium dayanum Rchb. ,Arundian graminifolia （D. Don） Hochr. ,C. lowianum （Rchb. f. ） Rchb. and C. tracyanum L. with 60^Co γ ray of different dose,to stat plant death rate, survival rate, survival plants＇ proliferation rate, observed variation rate of character and took regression analysis, the results showed： the half-lethiferous dose of Cymbidium dayanum Rchb. ,Arundian graminifolia （ D. Don） Hochr. , C. lowianum （ Rchb. f. ） Rchb. and C. tracyanum L. vitro plantlets were respective 20. 72Gy,26.31Gy,29. 88Gy,41.04Gy, and there were remarkable character mutation on treated plants such as： stems became dwarfer and stronger, leaves became broader some leaves could be seen the splits like saw tooth, have light spots on them and tortility.
出处
《云南农业大学学报》
CAS
CSCD
2007年第3期332-336,共5页
Journal of Yunnan Agricultural University
基金
国家自然科学基金项目(3060074)
云南省自然科学基金重点项目(2002C003P)
教育部春晖计划项目(Z-2005-2-65008)。
关键词
兰花
辐射诱变
半致死量
表型变化
Orchid
irradiation mutation
half-lethiferous dose
character mutation
