摘要
mRNA差异显示技术是在基因转录水平上研究差异表达和性状差异的有效方法之一。该技术以其简便、灵敏和RNA用量少,且可同时比较多组样品等优点成为目前该领域中最受青睐的技术之一。但是,该技术也存在假阳性率高、所得差异片段较短等诸多不足。针对这些缺陷,人们在引物的设计、PCR循环参数的优化、阳性克隆的鉴定等方面进行了改进。文章就该技术的原理、优越性、主要缺点、技术改进及其在寄生虫学研究中的应用方面进行了综述。
The mRNA differential display is a new technique to clone genes of differential expressing of the diversity organism. This technique is preferred in this research field currently because it owns some strong point such as simple, sensitive, few quantity of RNA,and can display differences from several samples. But it also has shortcomings, for example, the high rate of fake positive clones, the shorter differential fragments obtained. To overcome these limitations, several taches including total RNA, primers, the parameters of PCR and identification of clones have been modified. The principle,advantages,main limitations,modifications of this method and the application of this technique in parasitology have been reviewed in this article.
出处
《动物医学进展》
CSCD
2007年第5期61-65,共5页
Progress In Veterinary Medicine
基金
国家973前期计划项目(2006CB708512)
