摘要
目的:建立薄层扫描法测定微生物发酵转化液中熊果苷含量的方法。方法:微生物发酵转化液经过滤浓缩制备,以乙酸乙酯-乙酸-水(v乙酸乙酯:v乙酸:v水=3.1:1:1)为展开剂,检测波长570nm。结果:熊果苷在2～6μg范围内线性良好,相关系数r=0.9976。结论:本法快捷、简便、准确,可用于微生物发酵转化液中熊果苷含量的测定。
Objective： To establish a method for determination of the content of arbutin in microbe conversion solution by Thin-Layer chromatography. Methods： The microbe conversion solution was filtrated and condensed. The developing solvent was a mixture of ethyl acetate, acetic acid and water （3.1：1：1, v/v/v）. The detection wavelength was set at 570 nm. Results： The calibration curve was Lincar in the range of 2μLg-6μg with r value of 0.9976. Conclusions： The method was quick, simple and accurate for the determination of content of arbutin.
出处
《现代食品科技》
EI
CAS
2007年第5期76-78,共3页
Modern Food Science and Technology
关键词
熊果苷
发酵转化
薄层扫描
arbutin, microbe conversion, thin layer chromatography
