摘要
目的:构建人卵透明带蛋白(huZP3)基因原核表达载体。方法:利用PCR法扩增出含人卵透明带蛋白编码基因,先将其克隆到pGEM-T载体,进行序列测定和分析,然后将huZP3蛋白编码基因亚克隆至原核表达载体pGEX4T-1上,酶切鉴定。结果:获得一个核苷酸长度约为1200bp的基因,同源比较结果表明,与GenBank(NCBI:M60504)公布的ZP3基因序列有100%的同源性。酶切表明ZP3基因正确插入原核表达载体pGEX4T-1。结论:成功构建出含ZP3基因的原核表达载体。
Objective： To construct expression vector which can express human zona pellucida in prokaryotic cells. Methods： The ZP3 gene was amplified by PCR. The amplified DNA fragment was cloned into pGEM -T vector for sequencing analysis. The ZP3 gene was then subcloned into prokaryotic expression vector pGEX4T - 1. Results： A gene of 1200 bp was obtained and showed a 100% homology with ZP3 gene sequence published in GenBank （NCBI： M60504 ）. ZP3 gene was inserted into prokaryotic expression vector pGEX4T-1 correctly. Conclusion： ZP3 protion expression vector is constructed successfully.
出处
《中国妇幼保健》
CAS
北大核心
2007年第13期1792-1794,共3页
Maternal and Child Health Care of China
基金
国家自然科学基金资助项目(39870313)
关键词
人卵透明带蛋白
原核表达载体
构建
Human zona pellucida protein
Prokaryotic expression vector
Construction
