摘要
目的评价不同剂量125I籽粒近距离照射对神经胶质瘤干细胞的杀伤作用。方法术中取胶质瘤细胞,接种于含生长因子的无血清培养基中培养,使其中的脑肿瘤干细胞增殖,利用细胞免疫荧光检测脑肿瘤干细胞在细胞培养CD133的表达,将125I籽粒置入培养皿中,对脑肿瘤干细胞给予不同剂量、不同时间的近距离照射,检测CD133阳性细胞死亡率、增殖抑制的改变;通过细胞克隆形成法测定125I近距离照射对脑肿瘤干细胞的生长抑制作用。结果与空白对照组比较,照射组细胞死亡率,增殖抑制率明显升高;125I各实验组生长曲线抑制克隆形成,较空白组明显降低。结论125I近距离照射可以通过导致细胞死亡、抑制脑肿瘤干细胞增殖,在胶质瘤的治疗中发挥作用,具有进一步应用临床的前景。
Objective To evaluate the cytocidal effect of ^125I seeds brachytherapy on human glioma stem cells. Methods The tumor from patients undergoing tumor resections was dissociated, triturated into single cell and then filter, the cells were seeded into the serum-free medium, make the gliomas stem cells of gliomas cells proliferating. We perform immunocytochemistry of glioma stem cell for CD133 express. Then use different dose of ^125I seeds in the plate and culture together for 20,40,60 hours. Count the CD133 positive cells and observe the change of cells; determine the growth inhibition of the glioma stem cells by brachytherapy of ^125I seeds by the measure of cell clone formation. Results The dead cells in radiation group are obviously higher than that in control group. So does the growth inhibition. Conclusions Brachytherapy with ^125I seeds can kill the glioma stem cell and inhibit the proliferation in two groups, it shoud play an important role in the therapy of gliomas and have great perspective.
出处
《国际神经病学神经外科学杂志》
2007年第2期109-113,共5页
Journal of International Neurology and Neurosurgery
