摘要
目的研究伊班膦酸钠(IBN)对体外培养的人肺巨细胞癌高转移株PLA-801D细胞生长增殖的影响及其潜在的作用机制。方法采用MTT法检测不同浓度IBN、及相同浓度不同作用时间的IBN对PLA-801D细胞系生长增殖的影响,应用流式细胞仪检测不同浓度IBN作用下,PLA-801D细胞周期的变化。结果在10～100μg/ml的浓度范围内,IBN对PLA-801D细胞生长增殖均有抑制作用(P<0.01),抑制效果与IBN的药物浓度和作用时间呈正相关(r药物浓度=0.987,r作用时间=0.985);IBN可使细胞明显阻滞于S期,G2/M期细胞比例则明显减少。结论在10～100μg/ml的浓度范围内,IBN对体外生长的PLA-801D细胞的抑制作用呈剂量、时间依赖性,其机制可能与其将细胞阻滞在DNA合成期(S期),阻碍肿瘤细胞分裂增殖有关。
Objective To study the inhibitory effect of ibandronate （IBN） on the growth of lung cancer cell line PLA-801D in vitro, and the mechanism of such effect was studied. Methods The effects of different doses or treatment time of IBN on the growth of PLA-801D cell line were detected by MTT method. The mechanism of the effect was analyzed by the examination of cell cycle using flow cytometry. Remits With the concentration of 10 to 100 μg/ml, IBN obviously inhibited the growth of PLA-801D cell （P〈0. 01） in dose- and time-dependent manner （r concentration =0. 987, r treatment time =0. 985）, and blocked the cellular cycle at the S/G2 boundary. Conclusion With the concentration 10 to 100μg/ml, IBN can inhibit the growth of lung cancer cell line in dose- and time-dependent manner. Its mechanism probably relates to blockage of cell cycle at S phase.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2007年第4期352-354,共3页
Medical Journal of Chinese People's Liberation Army
