摘要
目的探讨羟基喜树碱(HCPT)对人口腔鳞癌细胞株KB细胞的凋亡诱导作用及其机制。方法体外培养KB细胞,MTT试验检测HCPT的细胞毒性作用;Hoechst33258染色和流式细胞术对细胞凋亡进行定性和定量分析;蛋白印迹检测Caspase-3和Bcl-2蛋白表达情况。结果HCPT能诱导KB细胞凋亡,且具有剂量依赖关系;随凋亡率增高,Caspase-3蛋白表达显著增强,Bcl-2蛋白表达显著减弱(P均<0.05)。结论HCPT可促进口腔鳞癌细胞凋亡,其可能机制是诱导Caspase-3蛋白表达和抑制Bcl-2蛋白表达。
[Objective]To study hydroxyeamptotheein（HCPT） inducing oral squamous cell carcinoma KB cell lines apoptosis and its mechanism.[Methods] KB cells cultured in vitro were treated with HCPT, MTT test was used to detect the eytotoxieity, hoeehst 33258 staining and flow cytometry（FCM） were used for qualitative and quantitative study of apoptosis, Western-blotting was used to detect the expressionn of easpase-3 and Bel-2 protein. [Rusults] HCPT could induce KB cells apoptosis in a dose-dependent manner. The higher apoptosis rate, the higher easpase-3 expression and lower Bel-2 expression （P〈0. 05）. [Conclusion] HCPT can induce the oral squamous cell eareinorna cell lines apoptosis, it may be related, with inducing the expression of easpase-3 and Bel-2 protein.
出处
《山东医药》
CAS
北大核心
2007年第11期24-25,共2页
Shandong Medical Journal
基金
聊城市科技局资助项目(2006-38)
关键词
羟基喜树碱
口腔鳞癌
细胞凋亡
hydroxyeamptotheein
oral squamous cell carcinoma
apoptosis
