摘要
目的了解肾综合征出血热(HFRS)患者PLT表面β3整合素CD61的表达水平及其与HFRS病情的关系。方法HFRS患者104例与正常埘照30例,应用流式细胞术测定CD61分子阳性PLT的百分率及PLT表面CD61的平均荧光强度(MFI).并按患者少尿期PLT表面CD61的MFI,将104例患者分为3组.进一步对PLT表面CD61表达水平与病情的关系.结合临床指标进行分析。结果HFRS患者发热期、少尿期、多尿期CD61刚性PLT的百分率与正常对照组之间差异无统计学意义.而发热期、少尿期和多尿期PLT CD61的表达水平(MFI分别为19.75±2.57、17.46±1.48和15.55±0.60)明显高于正常对照组(MFI为3.20±0.12)。少尿期HFRS患者PLT CD61表达水平与患者的PLT计数、血Alb水平呈负相关(相关系数分别为-0.637和-0.695),而与WBC、BUN、Cr和ALT水平呈正相天(相关系数分别为0.945、0.904、0.956和0.891);PLT CD61水平高表达组的PLT减少,而尿毒症、低白蛋白血症、肝功能异常和WBC升高的发生率则显著增高。结论HFRS患者PLT表面CD61的表达水平明显增高,且与患者的病期和病情严重程度密切相关,提示PLT CD61表达水平可反映HFRS患者病情严重程度,检测HFRS患者PLT CD61表达水平有助于监测患者病情。
Objective To investigate the relationship between the expression level of platelet membrane glycoprotein β3（GP Ⅲ a, CD61） and the severity of disease in patients with hemorrhagic fever with renal syndrome（HFRS）. Methods One hundred and four patients with HFRS and 30 healthy individuals were recruited. The percentage of CD61 positive platelets and the mean fluorescence intensities （MFI） of platelet membrane glycoprotein β3 were determined by flow cytometry （FCM）. The 104 patients studied were divided into three groups based on their expression levels of platelet membrane glycoprotein β3 at oliguric phase. Clinical data and laboratory parameters in different groups were compared and analyzed. Results The expression levels of CD61 in patients with HFRS were significantly higher than those in control group, although no significant difference in the percentage of CD61 positive platelets between patients with HFRS and controls was detected. The MFI of CD61 expression in patients with HFRS at fever phase, oliguric phase and polyuric phase was 19.75±2.57, 17.46±1.48 and 15.55±0.60, respectively, which was significantly higher than that in control group （3.20±0.12）. The expression level of CD61 in patients with HFRS at oliguric phase was negatively correlated with platelet count and serum albumin（r=-0. 637 and -0. 695, respectively） and positively correlated with white blood cell count, blood urea nitrogen, serum creatinine and alanine aminotransferase（r=0. 945, 0. 904, 0. 956 and 0. 891, respectively）. When the patients were compared according to the expression levels of CD61, it was indicated that the higher the expression level of CD61, the higher the incidence of uremia, hypoalbuminemia, abnormal liver function and leukocytosis. Conclusions The expression levels of platelet membrane glycoprotein β3 in patients with HFRS are different in different clinical phases and are significantly correlated with the severity of the disease in the patients. It suggests that the expression level
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2007年第3期152-155,共4页
Chinese Journal of Infectious Diseases
基金
国家自然科学基金(30271176)
关键词
肾综合征出血热
血小板
整合素Β3
血小板膜糖蛋白类
流式细胞术
Hemorrhagic fever with renal syndrome
Blood platelets
Integrin beta 3
Platelet membrane glycoproteins
Flow cytometry
