摘要
目的通过分析不同程度血糖状态与实验性糖尿病大鼠心肌胰岛素样生长因子1受体 mRNA(IGF-IR mRNA)表达数与心肌形态的关系,明确 IGF-1R mRNA 在糖尿病心肌病中的作用。方法制作糖尿病大鼠模型,于成模后3个月经光镜和透视电镜观察心肌的形态学改变。应用原位杂交技术对不同血糖状态下 IGF-1R mRNA 在心肌上的表达情况进行观察。结果 IGF-1RmRNA 在正常大鼠及糖尿病鼠(模型 B 组、C 组)心肌细胞膜和胞质中均有表达,其表达率分别为20%、30%和38%,且血糖及糖化血红蛋白 Al(HbA1c)水平与糖尿病大鼠心肌细胞膜及胞质 IGF-1RmRNA 表达数呈正相关,相关系数分别为0.869和0.865(均 P<0.01),表明与心肌形态结构改变相关。结论随着血糖水平升高,糖尿病大鼠心肌 IGF-1R mRNA 表达量增加,心肌形态结构改变加重,IGF-1R mRNA 表达量的变化很可能是糖尿病心肌病变发生、发展的重要因素。
Objective To investigate the role of insulin-like growth factor-1 receptor mRNA （ IGF- 1R mRNA） in diabetic cardiomyopathy. Methods Alloxan of the doses of 150 and 200 mg/kg was injected intraperitoneally into 20 male Wistar rats respectively so as to establish type 1 diabetes mellitus models （Groups B and C ）. The rats were used as normal controls （Groups A）. Three months later the rats were killed and blood samples were collected to undergo examination of plasma glucose, insulin, glycated hemoglobin （HbAlc）, and C peptide. The hearts were taken out to undergo light and transmission electron microscopy and in situ hybridization to detect the expression of IGF-1R mRNA in the myocardium. Results The levels of plasma glucose, HbAlc, insulin and C peptide of Groups B and C were all significantly higher than those of Group A （all P 〈 0.01 ） , and the levels of plasma glucose, HbAlc, insulin and C peptide of Group C being significantly higher than those of Group B too （ all P 〈 0.01 ）. Expression of IGF-1R mRNA was found in the myocardium of Groups A, B and C with the distribution areas of 20% , 30% , and 38% respectively. The expression of IGF-1R mRNA in myocardium was positively correlated with blood glucose and HbA1 c （ r = 0. 869, 0. 865, both P 〈 0.01 ）. Conclusion The increased expression of IGF-1R mRNA in the myocardium may play an important role in the progression of diabetic cardiomyopathy.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第18期1249-1251,共3页
National Medical Journal of China
关键词
糖尿病
心肌
受体
IGF
1型
模型
动物
原位杂交
Diabetes mellitus
Myocardium
Receptor, IGF type 1
Models, animal
In situ hybridization
