摘要
目的对结核分枝杆菌散在分布重复单位(mycobacterial interspersed repetitive units,MIRU)基因分型技术在山东省局部地区的应用进行评价。方法 2004年2月至2006年6月,对山东省12个县级结核病防治所实验室分离培养的826株结核分枝杆菌,应用 MIRU 基因分型技术进行分型。结果 826株结核分枝杆菌分为201种基因型,123个独特型,78个簇,最大簇基因型为223325173533。成簇的菌株中相对应的患者有流行病学接触史者18例。MIRU 基因分型法分辨率为0.90,位点26显示相对较高多态性。该基因分型法的重复性为100%。完成1株菌株基因分型需要成本人民币约50元。结论 MIRU 基因分型技术的重复性好,快速、简单、经济,不需要昂贵的设备。不足之处是223325173533基因型菌株在山东是社会传播的优势菌群,占分离结核分枝杆菌的30.89%,需要通过二线分型方法 IS6110限制性片段长度多态性基因分型技术或增加新的多态性较高的可变数目串联重复序列位点进一步提高分辨率。
Objective To evaluate the application of mycobacterial interspersed repetitive units （MIRU） in typing Mycobacterium tuberculosis in Shandong. Method 826 strains of M. tuberculosis from laboratories of 12 county tuberculosis dispensaries in Shandong were cultured and typed by MIRU genotyping in the KICID laboratory. Results The 826 strains were typed to 201 distinct MIRU patterns, and 123 isolates were unique, 703 strains were grouped into 1 of 78 different MIRU clusters, and the MIRU genotype of the largest cluster was 223325173533. Among the clustered strains, 18 patients had an epidemiological history of contact. The Hunter-Gaston discriminatory index was 0. 90. The allelic diversity of the sample was calculated for each other of the MIRU loci, and showed that MIRU26 had 10 alleles and was highly discriminative, while other five MIRU loci （MIRU31, 10, 39, 40, 4） were moderately discriminative. The reproducibility of the MIRU method was 100%. It took about RMB ￥50 to genotype one strain. Conclusions MIRU genotyping is a reproducible, fast, simple, and relatively cheap method. But because the isolates of 223325173533 genotype are predominant （30. 89% of the isolates ） in Shandong Province, it needs a second method IS6110 RFLP or by adding other more discriminative variable-number tandem repeat （VNTR） loci to improve the discriminative power.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2007年第5期355-358,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
山东省2006年度科学技术发展计划资金(2006GG2202001)
关键词
分枝杆菌
结核
基因型
串联重复序列
Mycobacterium tuberculosis
Genotype
Tandem repeat sequences
