摘要
目的观察盐酸氨溴索(AMB)注射液对吸烟引致大鼠慢性阻塞性肺疾病(COPD)的干预效果。方法2006年3月至8月,在哈尔滨医科大学第一临床医学院呼吸内科应用单纯吸烟法引致大鼠COPD模型,雾化吸入AMB进行干预。对各组进行支气管肺泡灌洗液(BALF)细胞计数;测定BALF中基质金属蛋白酶-9(MMP-9)、白细胞介素-8(IL-8)及肿瘤坏死因子-α(TNF-α)的质量浓度;定量分析肺平均内衬间隔(MLI)、平均肺泡数(MAN)、肺泡腔面积与总面积比(PAA)、肺内弹力纤维的相对面积。结果COPD组BALF中MMP-9、IL-8及TNF-α与BALF中白细胞总数、中性粒细胞百分比、中性粒细胞绝对计数均呈正相关;COPD组大鼠肺内弹力纤维受到严重破坏,MLI、PAA高于对照组,MAN和弹力纤维的相对面积低于对照组,BALF中MMP-9、IL-8及TNF-α的质量浓度均明显高于对照组;AMB组BALF中IL-8、TNF-α、MLI、PAA及MAN均介于其他2组之间,而弹力纤维的相对面积、MMP-9质量浓度与COPD组差异无显著性意义。结论单纯吸烟可导致大鼠COPD,气道和肺泡内的MMP-9、IL-8和TNF-α均可能参与了弹力纤维的破坏和COPD的形成过程。AMB在一定程度上可抑制COPD大鼠气道及肺泡内的IL-8和TNF-α释放。
Objective To observe interference effect of Ambroxol （AMB） hydrochloride injection on chronic obstructive pulmonary disease in rats caused by cigarette smoking exposure. Methods Chronic obstructive pulmonary disease （COPD） rat models were established by simple cigarette smoking exposure, interfered with AMB inhalation. Rats were divided into three groups. Cell counts were examined routinely in bronchoalveolar lavage fluid （BALF）. The concentrations of Matrix metalloproteinase-9 （MMP-9）. interleukin-8 （IL-8） and tumor necrosis factor-α （TNF-α） in BALF were measured. Quantitative analysis of pulmonary mean linear intercept （MLI）, mean alveolar numbers （MAN）, pulmonary alveolar area （PAA） and the relative area of elastic fibers were measured. Results Concentrations of MMP-9, IL-8 and TNF-α in BALF of COPD rats were positively correlated with total counts of white blood cell （WBC） ,percentage of neutrophil and absolute counts of neutrophil in BALF. Pulmonary elastic fibers were severely damaged in COPD rats; MLI, PAA were higher than those in control group （P 〈 0. 05 ） ; MAN and relative area of elastic fibers were lower than those in control group （P 〈 0. 05） ; concentrations of MMP-9, IL-8 and TNF-α were significantly higher than those in control group （P 〈 0. 01 ）. Concentrations of IL-8 and TNF-α, MLI,PAA and MAN in BALF of AMB rats were between COPD and control group,while relative area of elastic fibers and concentration of MMP-9 had no significant difference from those of COPD group （P 〉 0. 05）. Conclusion Simple cigarette smoking can cause COPD in rats. MMP-9, IL-8 and TNF-α in airway and alveoli pulmonum probably participate in the destruction of elastic fibers and forming of COPD. AMB inhalation can inhibit release of IL-8 and TNF-α in airway and alveoli pulmonum of COPD rats, alleviate the inflammation of local airway caused by cigarette smoking, playing a role in preventing and curing experimental COPD of rats.
出处
《中国实用内科杂志》
CAS
CSCD
北大核心
2007年第10期789-791,共3页
Chinese Journal of Practical Internal Medicine
基金
黑龙江省自然科学基金(D200535)
关键词
肺疾病
慢性阻塞性
盐酸氨溴索注射液
吸烟
Pulmonary disease, chronic obstructive
Ambroxol hydrochloride injection
Cigarette smoking
