摘要
目的探讨过氧化物酶体增殖物激活受体-γ(PPAR-γ)在兔动脉粥样硬化模型中的表达,以及阿托伐他汀的干预作用。方法2005年1月至2006年12月,在吉林大学中日联谊医院心内科将24只雄性日本大耳白兔随机分为高脂饮食组(A组)、高脂饮食+阿托伐他汀组(B组)和正常饮食组(C组)3组,每组8只。各组分别在喂养16周末处死,免疫组化法检测PPAR-γ,全自动生化分析仪检测血脂的表达水平。结果PPAR-γ的表达分别为A组(11·23±1·21)%,B组(11·05±1·02)%,C组(7·68±1·04)%,A、B2组比较差异无显著性意义(P>0·05),A、B2组与C组相比差异均有显著性意义(均P<0·01)。总胆固醇(TC)在A、B、C3组的表达分别为(23·51±10·58)mmol/L,(14·27±3·51)mmol/L,(1·36±0·33)mmol/L,各组间比较差异有显著性意义;低密度脂蛋白(LDL)在A、B、C3组的表达分别为(21·39±10·00)mmol/L,(14·23±4·01)mmol/L,(0·72±0·35)mmol/L,各组间比较差异亦有显著性意义;甘油三酯(TG)在3组间比较差异无显著性意义(P>0·05)。结论在兔动脉粥样硬化模型中PPAR-γ表达增高;阿托伐他汀可促进PPAR-γ的表达。
Objective To investigate the expression of peroxisome proliferator-activated receptor-γ（PPAR-γ） in a rabbit model of atherosclerosis, and the effect of atorvastatin, Methods Twenty-four male Japanese rabbits were separated into 3 groups（ 8 in each group）, which were high-fat diet group（ grgup A ）, high-fat plus atorvastatin group （group B ）and normal diet group（ group C）. All the animals were sacrificed after 16 weeks. The expression of PPAR-γwas detected with immunohistochemical assay, and plasma lipid with automatic biochemical analysis device. Results The expression of PPAR-γ were ,respectively,high-fat diet group（ 11, 23±1.21 ）%, high-fat plus atorvastatin diet group（ 11.05± 1.02）% and norreal diet group（7.68 ± 1.04）%. Between group A and B there wasn＇t any significant difference（P 〉0. 05）. Meanwhile, they both had significant differences compared with group C （ P 〈 0. 01 ）. TC and LDL expression both had significant differences among three groups ,while TG had no significant difference. Conclusion The expression of PPAR-γ increases in a rabbit model of atherosderosis;atorvastatin increases the expression of PPAR-γ.
出处
《中国实用内科杂志》
CAS
CSCD
北大核心
2007年第10期787-788,共2页
Chinese Journal of Practical Internal Medicine
基金
吉林省科技厅资助项目(200505173)
