摘要
目的:探讨染色体核型异常和DAZ基因缺失与生精障碍的关系。方法:应用多重PCR技术对60例无精、少精患者和60例正常生育力男性进行DAZ基因检测,并对60例无精、少精患者进行外周血染色体检查。结果:在60例无精、少精患者中共发现23例异常,约为38.3%(23/60),其中DAZ基因缺失4例,染色体异常19例,DAZ基因缺失率为6.7%(4/60),染色体异常率为31.7%(19/60),正常生育力男性未检测到DAZ基因缺失。结论:染色体异常和DAZ基因缺失与生精障碍密切相关,是无精、少精的重要原因。
Objective： To investigate the correlation of spermatogenesis impediment with chromosomal abnormality and with the deletion of DAZ gene. Methods： Included in the study were 60 azoospermic and oligozoospermic patients, as well as 60 fertile men as controls. MuIti-PCR was used to analyze the deletion of DAZ gene. Chromosomal quantity and construction were detected by G-band in the 60 patients. Results： Four of 60 patients showed deletion of DAZ gene and 19 patients showed chromosomal abnormality, the rate of DAZ deletion was 6. 7%（4/60） and the incidence of chromosomal abnormality was 31. 7%（19/60）. There was no deletion of DAZ gene in 60 normal fertile men.. Conclusion： Chromosomal abnormality and DAZ gene deletion are closely related to the spermatogenesis impediment,and they are important reasons for azoospermia and oligospermia.
出处
《陕西医学杂志》
CAS
北大核心
2007年第5期565-567,576,共4页
Shaanxi Medical Journal
基金
河北省科技厅技术攻关项目(No062761234)
