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RP-HPLC法测定黄柏中小檗碱、巴马汀和药根碱的含量 被引量:15

RP-HPLC determination of berberine, plmatine and jatrorrhizine in Huangbo
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摘要 目的:建立了用外标法对黄柏中小檗碱、巴马汀和药根碱同时定量的测定方法,考察小檗碱、巴马汀和药根碱的含量与其产地的关系。方法:高效液相色谱法,色谱柱:Kromasil-C_(18)(4.6mm×250mm,5μm),流动相:甲醇-乙腈-0.1mol·L^(-1)磷酸二氢钠(磷酸调pH为3.0)-2g·L^(-1)十二烷基硫酸钠-三乙胺(25∶25∶25∶25∶0.2),流速:1.0mL·min^(-1),检测波长:230nm,柱温:室温。结果:小檗碱、巴马汀和药根碱色谱峰面积与浓度呈良好的线性关系,线性范围分别是:盐酸小檗碱:21~495μg·mL^(-1),Y=3.144×10~6X+8.644×10~4,r=0.9999;盐酸巴马汀:2.0~40μg·mL^(-1),Y=7.570×10~5X+1.622×10~3,r=0.9999;盐酸药根碱:0.32~13μg·mL^(-1),Y=4.649×10~5X+2.161×10~3,r=0.9999。平均回收率小檗碱为98.0%,RSD为2.9%;巴马汀为97.7%,RSD为2.7%;药根碱为97.5%,RSD为2.4%。结论:本方法测定了21个不同产地的黄柏样品中小檗碱、巴马汀和药根碱含量,该方法分离度好,简便,重现性好。 Objective: A reserved - phase HPLC method was developed for the determination of and Berberine, Plmatine and Jatrorrhizine in Phellodendron chinense Schneid. or P. amurense Rupf. Whether there is a close relation between the content and their environment in which they are living was decided. Method :A Kromasil -C18 column (4. 6 mm×250mm,5μm)was used with the mobile phase being methanol- Aeetonitrile- 0. 1 mol·L^-1 mono- basic sodium phosphate (use the phosphoric acid adjust the pH at 3.0) -2 g ·L^-1 SDS -triethylamine (25:25: 25: 25:0.2 ,v/v) ,flow rate being 1.0 mL · min^-1 ,detecting wavelength being 230 nm,the column temperature being home temperature. All the 21 samples were determined. Result:The linear response range from :20 -495 μg·mL ^- 1 of berberine, Y = 3. 144 x 106X + 8. 644× 10^4 ( r = 0. 9999 ) ; 2.0 - 40 μg·mL ^- 1 of palmataine Y = 7. 570 × 10^5X + 1. 622 × 10^3 ( r = 0. 9999 ) ;0.32 - 13 μg·mL ^- 1 of jatrorrhizine, Y = 4. 649 × 10^5X + 2. 161 ×10^3 ( r = 0. 9999 ) ;The recoceries of berberlne were 98.0% , RSD was 2.9 % , the recoveries of palmataine were 97.7 %, RSD was 2. 7% and the recoveries of jatrorrhizine were 97.5% ,RSD was 2.4%. Conclusion:All 21 samples were determined by HPLC. The method is quick and simple with good repetition.
作者 王瑾
出处 《药物分析杂志》 CAS CSCD 北大核心 2007年第4期610-612,共3页 Chinese Journal of Pharmaceutical Analysis
关键词 小檗碱 巴马汀 药根碱 高效液相色谱法 含量测定 Huangbai Berberine palmataine jatrorrhizine HPLC
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