摘要
目的:研究蛹虫草中类胡萝卜素的提取工艺。方法:采用正交试验法进行优选,分光光度法测定类胡萝卜素含量。结果:酸热法对蛹虫草中类胡萝卜素的提取有显著性影响。结论:最优提取组合为盐酸浓度1mol/L、盐酸用量15mL/g、酸浸时间20min、热处理时间4min,丙酮是从蛹虫草中浸提类胡萝卜素较优越的有机溶剂;在此浸提条件下,蛹虫草中类胡萝卜素含量可达2158μg/g干样品。
Objective： The extractions on the carotenoid produced from Cordyceps militaris were studied. Methods： The content of carotenoid was determined by UV-spectrophotometry. The optimum extraction process was selected with the orthogonal design. Result： The experimental results showed that the method combining hydrochloric acid（HCI） with heating（by boiling water） was suitable for extraction on the carotenoid produced from Cordyceps militaris. Conclusion： The optimal extraction condition was obtained as follows： HCI concentration 1mol/L, HCI addition 15mL/g（dry sample）, soaking 20min and heating 4min. Acetone was the optimal extracting organic solvent. Under the above optimal conditions, the yield of carotenoid could reach 2158μg/g dry sample.
出处
《食品科技》
CAS
北大核心
2007年第4期99-103,共5页
Food Science and Technology
关键词
蛹虫草
类胡萝卜素
正交试验
Cordyceps militaris
carotenoid
orthogonal test
