摘要
在组织培养过程中诱导苜蓿体细胞胚发生的关键因素有两个：一是植物材料，包括基因型和外植体类型；二是培养基成分。研究结果认为：紫花苜蓿体细胞胚发生能力较强，无菌苗子叶和下胚轴是发生率较高的外植体；2．0mg／L的2,4-D补加适宜浓度的NAA、KT和BA等激素是相对平衡的激素组合，也是必需的。添加一些有机物有利于提高体细胞胚发生率。
There are two key factors inducing alfalfa embryos occurrence during tissue culture. One is plant material including genotype and explants type; another is culture medium component. The research result showed that alfalfa embryos occurrence ability is stronger, cotyledon with germfree seedling and the hypocotyl are external plant tissue type that has higher occurrence. It is necessary to append NAA, KT and BA with fitting concentration on 2,4-D with 2.0 mg/L.h is propitious to boost the embryos occurrence to append some organic matter.
出处
《牧草与饲料》
2006年第1期12-14,31,共4页
Journal of Forage & Feed
