摘要
目的:探讨溶血磷脂酰胆碱(lysophosphatidylcholine,LPC)对牛视网膜微血管内皮细胞(bovine reti-nal microvascular endothelial cells,BRECs)一氧化氮(nitric oxide,NO)和一氧化氮合酶(nitric oxide synthase,NOS)生成的影响。方法:原代培养的牛视网膜微血管内皮细胞分为4组:对照组,LPC(20μmol/L、40μmol/L、60μmol/L)3组;LPC各组根据不同作用时间(6 h、12 h、24 h)又分为3个亚组,用NO和NOS试剂盒检测各组内皮细胞培养上清液中NO含量和NOS活性。结果:LPC可使BRECS条件培养基中NO含量逐渐减少,NOS活性逐渐减弱,具有时间和剂量依赖性,LPC(60μmol/L)作用24 h时效果最明显,差异有统计学意义(P<0.05)。结论:LPC可能通过抑制BRECS生成NO和减弱NOS活性而促进糖尿病视网膜病变的发生发展。
Objective： To study the effects of lysophosphatidylcholine （LPC） on the production of nitric oxide （NO） and nitric oxide synthase （NOS） in cultured bovine retinal microvascular endothelial cells （BRECs）. Methods： The primary BRECs were divided into 4 groups： the control group and 3 LPC groups according to the doses used （20 umol/L, 40 umol/L, and 60 umol/L）. Each LPC group was divided again into 3 subgroups depending on incubation time. NO production and NOS activity in conditioned media in which BRECs were cultured were assessed with NO and NOS kits. Results： In LPC groups, NO production and inhibited NOS activity decreased significantly （ P 〈 0.05）. The changes showed time and dosage dependence. Conclusion： LPC may play an important role in the development of diabetic retinopathy through inhibiting activity of NOS and decreasing NO production.
出处
《贵阳医学院学报》
CAS
2007年第2期142-145,共4页
Journal of Guiyang Medical College
