摘要
为探讨乙型肝炎(简称乙肝)疫苗对宫内乙肝病毒(HBV)感染的作用机理,应用含HBV辅助性T淋巴细胞抗原表位的合成肽(前S_2 120～145)为刺激原,观察18例宫内HBV感染婴儿外周血特异性淋巴细胞增殖反应。结果表明,宫内HBV感染组刺激指数(SI)为1.5±0.4,明显低于急性HBV感染组(SI=3.7±1.4)。其中宫内HBV感染对乙肝疫苗无反应组SI为1.2±0.4,明显低于宫内感染接种疫苗有效组(SI=1.74±0.4)。结合血清抗HBs滴度,表明宫内HBV感染婴儿在T淋巴细胞水平对HBV抗原耐受,其耐受程度与抗HBs的产生和滴度有关。
In order to clarify the mechanism of hepatitis B virus (HBV) vaccine in protecting in- trauterine HBV infection, we observed the proliferation of specific lymphocyte in the peripheral blood of 18 HBV infected newborns, using synthetic peptide (pre S_2 120～145)containing T helper lymphocyte epitope as stimulus. The results showed that stimulation index (SI) (1.5±0. 4) was much lower in the intrauterine HBV infection group than in acute infection group(3.7±1.4). The SI of non-responders to HBV vaccine in the intrauterine HBV infection group (1.2±0.4) was apparently lower than that of responders(1.7±0.4). This fact, in conjunction with serum anti-HBs level indicates that the T lymphocyte of the newborns with the HBV-intrauterine infection has immune tolerance or partial immune tolerance to HBV antigenic epitope, the level of which is related to the response to HBV vaccine.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1997年第1期26-28,共3页
Chinese Journal of Pediatrics
基金
国家自然科学基金
上海市科技发展基金
关键词
乙型肝炎
妊娠并发症
淋巴细胞转化
宫内感染
Hepatitis B Pregnancy complications, infections Lymphocytes transformation
