摘要
目的观察七氟醚对兔肺缺血再灌注损伤超微结构的影响,并探讨其可能机制。方法72只日本大耳白兔,随机均分为4组(n=18)假手术组(S组)、缺血再灌注组(IR组)、七氟醚-缺血再灌注组(Sev-IR组)和七氟醚组(Sev-S组)。S组开胸游离左肺门后,未行缺血再灌注。IR组、Sev-IR组参照Ep-pinger方法建立肺缺血再灌注模型,Sev-IR组吸入30min1肺泡最小有效浓度(MAC)七氟醚后行缺血再灌注,Sev-S组吸入30min1MAC七氟醚后不进行缺血再灌注。分别在缺血45min、再灌注60、120min处死兔,使用电子显微镜观察各组不同时间点肺组织超微结构的变化。并在再灌注120min测各组髓过氧化物酶(MPO)的活性。结果电镜下IR组肺泡毛细血管内皮细胞、Ⅰ型肺泡上皮细胞水肿变性,肺泡壁增厚,Ⅱ型肺泡上皮细胞膜微绒毛减少,线粒体肿胀、嵴减少,且胞质中板层小体明显减少,肺间质水肿,毛细血管腔内中性粒细胞(PMN)堵塞。Sev-IR组毛细血管内PMN附壁减少,Ⅰ型肺泡上皮细胞内吞饮小泡增加,Ⅱ型肺泡上皮细胞表面微绒毛增多。在再灌注120min时,IR组和Sev-IR组肺组织MPO的活性明显高于S组(P<0.01),而Sev-IR组MPO的活性低于IR组(P<0.05)。结论七氟醚对兔肺缺血再灌注损伤引起的超微结构改变有一定改善作用,其机制可能与降低PMN在肺脏的聚集有关。
AIM： To investigate the effects of sevoflurane inhalation on the ultrastructure changes in lung tissue during ischemia-reperfusion lung injury in rabbits and explore the underlying mechanisms. METHODS： Seventy two Japanese long-ear white rabbits weighing 2.5 - 5.0 kg were randomly divided into four groups （n = 18）： sham operation group S; IR group in which hilum of left lung was clamped for 45 min and then unclamped for reperfusion for 120 min; Sev-IR group in which 1MAC sevoflurane was inhaled for 30 min before ischemia; Sev-S group 1MAC in which sevoflurane was inhaled for 30 min without IR. The uhrastmcture changes in lung tissue were observed under electron microscopic at 45 min after pulmonary ischemia, 60 and 120 min of reperfusion. Myelopemxidase of lung was measured at 120 min of reperfusion. RESULTS： In group IR： pnemnonocyte Ⅰ and vascular endothial cell became swollen and deteriorated; there were reduced microvilli on the surface of pneumonocyte Ⅱ , and Ⅱ type alveolar epithelial cells were swollen or vesicular and plenty of polymorphonuclear neutrophils stayed in capillary chambers. The increased levels of myeloperoxidase in group IR and group Sev-IR were significantly higher than those in group S, but compared with group IR, group Sev-IR could inhibit the increase of myeloperoxidase after 120 min of reperfusion （ P 〈 0.05）. CONCLUSION： Sevoflurane attenuates the IR-induced pulmonary ultrastructural changes, possibly through an inhibition of polymorphonuclear neutrophil recruitment into the lung.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2007年第3期313-316,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
七氟醚
肺
再灌注损伤
超微结构
sevoflurane
lung
reperfusion injury
ultrastructure
