摘要
为更好地了解勤奋金属球菌(Metallosphaera sedula)作用下沉淀的累积进程及沉淀的特性,将一株在我国首次分离鉴定的勤奋金属球菌(YN23)接种在以Fe^(2+)为能源的培养基中,于该菌最佳生长条件(pH1．5,53℃,0．2g·l_(-1)酵母提取物,30g·1_(-1)Fe_2SO_4·7H_2O and 170rpm)下培养。接种25h后,当Fe^(2+)氧化率达到90%时,开始出现沉淀,pH也达到1．92的最高值;到第95h,当沉淀累积到7．9g·1^(-1),时,沉淀反应停止,此时pH达到1．32的最低点。菌群密度随着Fe^(2+)的氧化,前期快速增长;当沉淀出现以后,随着沉淀的累积,逐渐降低。X衍射图谱、红外吸收光谱、能谱和扫描电镜数据揭示,YN23菌株合成的沉淀是黄钾铁矶和黄铵铁矾的混合物,形态特征更接近于后者。
To have a better understanding on the evolution of MetaUosphaera sedula-mediated precipitation and the properties of the precipitate synthesized by this species, a newly identified extremdy thermophilic strain （YN23） of MetaUosphaera sedula was cultured in the medium comaining Fe^2＋ as energy resource under optimal conditions （pH 1.5, 65℃, 0.2g·1^-1 yeast extract, 30g·l^-1 Fe2SO4·7H2O and 170rpm）. XRD, EDS, FIIR and SEM reveal the precipitate obtained from YN23-inoculated flasks to be a mixture of potassium jarosite and ammoniojarosite, with morphological features similar to the latter. Precipitation was first detected once over 90% of Fe^2＋ was oxidized at hour 25 with a peak pH value of 1.92, and was ended when precipitate reached the highest point of 7.9g·l^-1 at hour 95 with the lowest pH value of 1.32 in solution. Microbial density underwent a rapid increase along with Fe^2＋ oxidation and a gradual decrease with precipitate piling up.
出处
《现代生物医学进展》
CAS
2007年第4期481-485,共5页
Progress in Modern Biomedicine
基金
Foundation item:Chinese Science Foundation for Distinguished Group(50621063)
National Basic Research Program(973 Program)of P.R.China(2004CB619201)
