摘要
根据猪SRY基因序列设计合成2对巢式PCR引物作为性别鉴定引物,根据猪β-珠蛋白基因序列设计了一对引物作为内标引物建立了猪胚胎性别鉴定的PCR反应体系。公猪可以扩增出187bp的SRY基因片段和255bp的β-珠蛋白基因片段,母猪只能扩增出255bp的β-珠蛋白基因片段。由于巢式PCR只需3～8个细胞就可以在紫外灯下看到扩增结果,而常规PCR则需要较多的细胞,所以胚胎性别鉴定时使用巢式PCR效果更好。
We designed 2 pairs of SRY gene nested primers for sex determination and a pair of β-globin gene primer as an internal standard in this study and established the system of PCR.As a result, 187 bp and 255 bp bands could be visible on the gel in ultraviolet transiUuminator for male , but for female ,only 255 bp band could be visible. It needed only 3 to 8 cells for obtaining amplification result by nested PCR, but more cells were needed by normal PCR. So we could obtain better results of sex determination of bovine preimplantation embryos by nested PCR.
出处
《畜禽业》
2007年第5期14-17,共4页
Livestock and Poultry Industry
