摘要
定量研究了随柠檬酸包覆纳米磁性氧化铁粒子浓度的增加，两种不同的细胞系（HeLa及HEK293）中细胞数目百分比的变化情况。细胞与纳米磁性粒子在37℃及5％CO2气氛中培养24小时。通过实验确定了纳米氧化铁粒子对两种细胞系的临界浓度c，即超过该浓度细胞出现大量死亡。对HeLa细胞，Фc=3×10^-4（1．5mg／mL）；对HEK293细胞，Фc=7×10^-4（0．35mg／mL），显示了与HEK293细胞相比，HeLa细胞可耐更高浓度的纳米氧化铁粒子。这些临界浓度为纳米磁性氧化铁粒子在生物医学中的应用提供了安全剂量的上限。
Quantitative studies is reported on the percentage change in the number of cells as a function of concentration of magnetic nanoparticles, citrate-coated iron oxide nanoparticles. The incubation is conducted at 37℃ in 5% CO2 atmosphere for 24 hours. A critical value of particle volume fraction, Фc was found, above which appreciable number of cell death occurs. This critical value differs in two different cell lines with Фc=3×10^-4（1.5mg/mL） for HeLa cell line and Фc=7×10^-4（0.35mg/mL） for HEK 293 cell line, indicating that HeLa cells are more robust against the magnetic nanoparticles. These critical values give the upper limit for safe dosage beyond which the toxicity sets in for the biomedical applications of magnetic nanoparticles.
基金
This work was supported by the National Natural Science Foundation of China under grant No. 50672039.
