摘要
目的探讨以简便、高效的方法获取血管平滑肌细胞,为相关研究提供实验材料。方法采用组织贴块法进行原代培养,胰酶消化法传代,并应用相差显微镜和平滑肌肌动蛋白对细胞进行形态学和免疫组化鉴定。结果90%的组织块接种存活,培养5代的平滑肌细胞纯度达98%以上。镜下可见培养细胞呈典型的“峰-谷状”样生长,免疫组化染色显示胞浆内平滑肌肌动蛋白阳性表达。结论本法简便、可靠,短期内可获大量高纯度、功能良好的血管平滑肌细胞,可作为研究心血管疾病良好的体外模型。
Objective To approach the method that produced high purified vascular smooth muscle cells （VSMC） from rat thoracic artery in short term for related researches. Methods The substrate-attached explants method was applied for cell culture of rat thoracic artery. Morphology of the cells was observed by inverted micro- scope and identified by immunohistochemical methods employed smooth muscle-α-actin. （α-SM-actin）.Results 90% of the cultured explants survived, the purity of fifth passage VSMC was more than 98%. The passaged VSMC developed typically ＂peak and valley＂ growth pattern and expressed the smooth muscle specific differentiation marker α-SM-actin. Conclusion The method presented here is a simple and effective method to isolate and purify smooth muscle cells from rat thoracic artery for cardiovascular researches.
出处
《中国心血管病研究》
CAS
2007年第5期363-365,共3页
Chinese Journal of Cardiovascular Research
基金
山西省卫生厅科技攻关项目(200622)
关键词
肌
平滑
血管
细胞
培养的
大鼠
主动脉
Muscle,smooth,vascular
Cells, cultured
Rats
Aorta
