摘要
研究了利用异丙醇破壁、丙酮二次纯化提取SOD生产工艺,最佳提取条件为:异丙醇浓度为90%、时间120min、pH7.0,粗酶液的收率为73%,所得SOD的酶比活为3048.7u/mg。与胞内SOD提取老工艺相比,具有工艺简单、设备少、操作简便、成本低等特点。(孙悟)
The extraction techniques of SOD （cell wall broken by isopropyl alcohol and secondary purification by propanone） were investigated. The best extraction conditions were as follows： 90 % isopropyl alcohol concentration, extraction time 120 min, pH value as 7.0, the yield of crude enzyme liquid as 73 %, and the specific enzymatic activity of SOD was 3048.7 u/mg. Compared with traditional intracellular SOD extraction techniques, the method had the advantages of simple techniques, less apparatus, simple operation and low cost etc.（Tran, by YUE Yang）
出处
《酿酒科技》
北大核心
2007年第5期89-91,共3页
Liquor-Making Science & Technology
关键词
综合利用
啤酒酵母
SOD
提取
comprehensive utilization
waste beer yeast
superoxide dismutase（SOD）
extract
