摘要
采用酶抑制法和AR—CM－MIC阳离子测定系统观察了不同剂量He－Ne激光对小鼠红细胞SOD活性及单个人淋巴细胞、大鼠神经细胞内游离钙浓度的影响．实验结果表明低强度激光血管内照射（ILLLI）能提高小鼠红细胞SOD活性，6.75×10~3J／cm^2天的照射剂量作用最明显；功率6mW，持续5分钟的He－Ne激光能提高高体淋巴细胞和神经细胞的细胞内钙浓度且不影响细胞形态，提示激光的生物刺激效应似有一最适刺激参数。
Superoxide dismutase (SOD) activity of erythrocyte in mice and intracellular free calcium concentration([Ca2+]i) of single human lymphocyte and rat neuron were investgated before and after low level laser irradiation by use of SOD inhibiting method and Ca2+ sensitive indicator Fura- 2 with Spex AR-CM -MIC Cation Measerment System. The findings suggest that intravascular low level laser irradiation (ILLLI) enhanced SOD activity of mouse erythrocyte, and the effects induced by 6. 75 X 103J/cm2/day were better than those did by 2. 70X104J/cm2/day. It seemed that irradiation with optimum constants would result in the best effect on organism.
出处
《应用激光》
CSCD
北大核心
1997年第2期89-93,共5页
Applied Laser
基金
国家自然科学基金
南京大学国家配位化学重点实验室资助
关键词
低强度
血管内照射
细胞
SOD
氦氖激光
low level He-Ne laser, intravascular irradiation, mouse erythrocyte, superoxide dismutase, calcium, human lymphocyte, rat neuron
