摘要
目的评价浸润淋巴细胞（ＴＩＬ）对原发性肝癌患者预后的影响。方法应用真彩色图像分析、免疫组织化学、３Ｈ－ＴｄＲ掺入试验和乳酸脱氢酶释放测定等方法，检测新鲜手术标本ＴＩＬ细胞的组织密度、活化增殖和细胞毒功能，并进行临床随访。结果按癌灶内ＴＩＬ浸润程度本组患者分为无浸润、低度浸润和大量浸润三类。肿瘤临床类型不同，ＴＩＬ浸润程度亦各异；无ＴＩＬ浸润组患者，术后生存时间较有浸润组短，术后肝癌复发率也高于后者。新鲜分离的ＴＩＬ细胞的活化增殖和杀伤活性均低于自体淋巴细胞（ＰＢＬ），激活后对自体肝癌细胞产生高效杀伤作用。结论原发性肝癌患者ＴＩＬ细胞与预后有关。
Objective To determine whether tumor infiltrating lymphocytes(TIL)could affect the prognosis for patients with primary hepatic carcinoma(PHC). Methods Tissue distribution,proliferative property and cytotoxicity of TIL were measured in colour medical image analysis,immunohistochemichemical technique,3H thymidine proliferative response and LDH release assay. Results On the basis of infiltrating level of TIL,all patients with PHC were classified into three types in which there can be non infiltrating,lower infiltrating and high infiltrating.The survival time of patients without TIL was short erthan that of patients with TIL.In addition,their postoperative intrahepatic recurrences were higher than those of the latter.Freshly isolated TIL showed more poor proliferation and cytotoxicity than autologous peripheral blood lymphocytes(PBL)in vitro.TIL activated with anti CD3McAb and rIL 2display edhigher cytotoxicity against fresh autologous hepatic carcinoma cells than against K562 targets. Conclusion Clinically,TIL are associated with the prognosis for patients with PHC,however,it is in the tumor nest that the functions of TIL are impaired or suppressed by some factors locally produced by tumor cells in vivo.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第3期167-170,共4页
National Medical Journal of China
关键词
淋巴细胞
肿瘤浸润
免疫学
肝细胞癌
预后
lymphocytes,tumor infiltrating Cytotoxicity,immunologic Carcinoma,hepatocellular
