摘要
目的:研究黑木耳(Auricularia auricula)“黑29”的漆酶纯化,为进一步酶学性质的开展、酶应用和酶基因克隆提供理论基础。方法:采用硫酸铵分级沉淀和柱层析技术分离蛋白,通过PAGE和SDS-PAGE电泳检测蛋白。结果:SDS-PAGE电泳检测发现粗酶液含三种漆酶,分子量大小分别为LacA(60kD)、LacB(34kD)、LacC(19kD);纯化后获得纯化的单一漆酶LacA、LacC组分。结论:得漆酶两个单一组分,为进一步漆酶研究奠定基础。
Objective：In order to study enzymatic properties and enzymatic gene colong,purification of laccase by Auricularia auricula was studied in the paper.Methods：The laccase from Auricularia auricula ＂Hei 29＂ was purified by two steps including treatment of（NH4）2SO4 and chromatography on DEAE-Sephacel.Results：PAGE and SDS-PAGE for laccase crude extract showed that three kinds of isoenzymes exist in Hei 29.The molecular weight of the three isoenzymes was determined by SDS-PAGE： LacA（69kD）,LacB（34kD）,LacC（19kD）.The LacA and LacC were purified by ammonium sulphate fractionation, DEAE - Sephacel chromatography. Conclusion： The study gained two purifications：LacA and LacC.
出处
《生物技术》
CAS
CSCD
2007年第2期55-57,共3页
Biotechnology
基金
黑龙江省青年科学技术专项基金项目资助("黑木耳一种重要蛋白质(酶)的分析研究"
编号:QC05C62)
关键词
黑木耳
漆酶
同工酶
纯化
Auricularia auricular
laccase
isoenzymes
crude extract
