摘要
以超速离心法提取的HepG2细胞总RNA为模板，应用RT-PCR技术扩增得到编码人干细胞因子（SCF）全长CDNA（约0.8kb）。克隆、序列分析确证编码人SCF膜外活性区的CDNA（约0.5kb）结构正确，构建了含有该基因的表达质粒（PBV-mhSCF）并在大肠杆菌中获得高效表达。
The total RNA of HepG2 cell was extracted as the template by ultrocentrifuge method The full length cDNA (0. 8 kb) encoding the human stem cell factor (hSCF) was amplifie by RT-PCR method. The cDNA encoding mature hSCF (0.5 kb) was sequenced and was recombined into the expression vector (PBV-220). The expression level of rhSCF in E. coli DH5a was about 15% of the total protein.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1997年第1期29-34,共6页
Acta Academiae Medicinae Sinicae
基金
中国医学科学院基金
