摘要
从小麦粉中提取植物酯酶，用70％饱和硫酸铵对植物酯酶进行纯化，酶催化反应温度在45℃、pH4．5～5．5时酶活性最大，酶催化反应动力学显示10min内反应呈线性关系。利用有机磷农药能抑制植物酯酶酶促反应的机理，建立了快速测定粮食中有机磷农药残留的方法。测定有机磷农药残留反应体系最大吸收峰是在480nm之间，最适乙酸-β-萘酯用量为1．0mL；在农药浓度在0．1～1．0mg／kg之间时，所到之处得到的吸光值与农药浓度呈线性相关；在青菜汁、茶叶汁和大米提取液回收率分别为89％、84％和93％。
The plant - esterase extracted from wheat flour was purified by 70% saturated ammonium sulfate. The obtained plant -esterase exhibits its maximum activity at 45℃ and pH 4.5 - 5.5, and exhibits a line relation of reactive velocity vs reactive time within 10 min. A rapid detection method of organophosphorus pesticide residues was developed on the following principle ： the organophosphorus pesticide can inhibit the plant - esterase activity. The reaction system of the organophosphorus pesticide determination shows the following characters ： maximum peak on 480 nm, optimal dosage of acetic-β -naphathone 1.0 mL, line relation of absorption value vs organophosphorus pesticide concentration in the pesticide concentration range of 0.10 - 1.0 mg/kg, recovery rate of 89%, 84%, and 93% for China vegetable juice, tea juice, and extract of rice, respectively.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2007年第2期8-11,共4页
Journal of the Chinese Cereals and Oils Association
基金
江苏省科技厅农业高新技术产业项目(BE2003345)
江苏省教育厅高新技术产业项目(JHB04-003)
关键词
小麦粉酯酶
纯化
有机磷农药
快速测定
plant -esterase from wheat flour, purification, organophosphorus pesticide, rapid determination
