摘要
Marker-assisted selection has become an integral component of many crop breeding programs in both the private and public sectors throughout the world. Various markers, such as RFLP, RAPD, AFLP and SSR are
Marker-assisted selection has become an integral component of many crop breeding programs in both the private and public sectors throughout the world. Various markers, such as RFLP, RAPD, AFLP and SSR are being used by the crop breeding community. The advent of high throughput sequencing technology has generated an enormous amount of DNA sequence information for the genomes of many species of economic importance. This includes the draft genome sequences of two subspecies of rice, one of the most important food crops of the world and more than 40 million expressed sequence tags （EST） in the public databases. This wealth of information provides the opportunity to develop novel approaches to discover markers such as single nucleotide polymorphism （SNP） and targeted local lesions in genomes （TILLING） techniques, which are being applied to different crops at a fast pace. The TRAP （targeted region amplification polymorphism） marker technique is a fairly new, relatively high throughput, persuasively reliable, and broadly applicable fingerprinting technique. TRAP harnesses existing sequence information and leverages bioinformatics tools to design fixed primers against known sequences of annotated ESTs,
出处
《分子植物育种》
CAS
CSCD
2007年第2期251-251,共1页
Molecular Plant Breeding
关键词
分子标记
农作物
开发
基因多态性
TRAP （targeted region amplification polymorphism） marker, Gene tagging, Genome mapping
