摘要
纯化的黑曲霉Aspergillus niger WM20-11所产的酸性β-甘露聚糖酶经Sephadex G-100凝胶过滤层析和SDS-PAGE分别测得相对分子质量为39 000和40 000;IEF-PAGE测得酶的等电点为4.0;最适作用pH值和温度分别为3.5和70℃;糖质量分数19.6%;Mg2+、Ca2+、Li+、Na+、K+对酶有激活作用,Pb2+、Co2+、Fe3+、Mn2+、Hg2+对酶有抑制作用;酶对角豆胶的Km和Vmax分别为66.7 g/L和333μmol/(min.mg);酶蛋白的氨基酸组成中Asp和Glu含量最高;HPLC分析角豆胶酶水解液的主要产物是低聚糖。
In this manuscript, the characterization of acidic β-mannanase from Asp. niger WM20- 11 was investigated. Molecular weight of the enzyme was determined as 39 000 on Sephadex G- 100 gel filtration and 40 000 on SDS-PAGE. The isoelectric point was estimated to be 4.0 by IEF-PAGE. The optimum temperature and pH were 70 ~C and 3. 5. It contained 19. 6% carbohydrate. The activity of the enzyme was stimulated by Mg^2＋, Ca^2＋, Li^＋, Na^＋, K^＋, but inhibited by Pb^2＋ ,Co^2＋ ,Fe^3＋ ,Mn^2＋ and Hg^2＋. Km and Vmax were 66.7 g/L and 333 μmol · min^-1·mg^-1 with locust bean gum. The content of Asp and Glu was the highest. Enzyme hydrolysis products from locust bean gum were oligosaccharides by HPLC analysis.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2007年第2期21-25,共5页
Journal of Food Science and Biotechnology
基金
国家"九五"重大科技攻关项目(96-C03-02-01)
关键词
黑曲霉
Β-甘露聚糖酶
性质
Aspergillus niger
β-mannanase
Characterization
