摘要
目的在细胞水平证实中药余甘子(Phyllanthus Emblica L.)的两个可溶性鞣质成分Phy-13(beta-1-O-galloyl-3,6-(R)-hexahydroxydiphenoyl-d-glucose)和Phy-16(1,6-di-O-galloyl-beta-d-glucose)是否具有抗动脉粥样硬化作用。方法人脐动脉内皮细胞ECV-304经ox-LDL(50 mg/l)诱导,MDA试剂盒检测Phy-13和Phy-16作用后内皮细胞培养上清液中丙二醛(MDA)的水平,MTT法检测动脉血管平滑肌细胞(VSMC)的增殖,细胞计数仪计数单核细胞和内皮细胞的黏附。结果Phy-13和Phy-16能减少内皮细胞MDA的生成,减少ox-LDL诱发的单核细胞和内皮细胞黏附,并能对抗ox-LDL诱导的动脉血管平滑肌细胞(VSMC)增殖。结论Phy-13和Phy-16能够对抗ox-LDL诱导的动脉粥样硬化,这可能是余甘子治疗动脉粥样硬化相关疾病的重要机制之一。
Objective To clarify the mechanism of antiatherogenic effects of Phyllanthus emblica L., which is widely used in treatment of atherosclerosis- related diseases and associated with ox- LDL by the soluble tannin, Phy- 13 （beta- 1 - O- galloyl- 3, 6 - （R） - hexahydroxydiphenoyl- d- glucose）, and its analogue Phy- 16 （1, 6 - di- O- galloyl- beta- d- glucose）. Methods Human arteria umbilical endothelial cells, ECV - 304, were incubated with ox - LDL （50 mg/l）, treated with corilagin or Dgg16 at different doses, and then incubated with monocytes. Malondialdehyde （MDA） in the culture media was determined and the number of monocytes adhering to ECV- 304 cells was counted cytometrically. The rat vascular smooth muscular cells （VSMC） were incubated in media with or without ox - LDL （50 mg/l）, and with Phy- 13 or Phy- 16 at different doses too, and the proliferation of them was assayed by MTT method. Results Both Phy- 13 and Phy- 16 were able to reduce MDA content, prevented ECV - 304 cells from being adhered by the monocytes, and inhibited VSMC proliferation that induced by ox - LDL by alleviating oxidation injury for treating atherosclerosis. Conclusion These two compounds are effective in inhibiting the progress of atherosclerosis or by inhibiting ox- LDL- induced VSMC proliferation, which may be the promising mechanisms for treating atherosclerosis.
出处
《实用预防医学》
CAS
2007年第2期352-355,共4页
Practical Preventive Medicine
