摘要
目的本实验设计合成靶向核因子κB(NF-κB)的哑铃形圈套寡核苷酸(ODNs),并验证其对白介素10(IL-10)的作用。方法以阳离子脂质体为载体,将圈套ODNs转染大鼠pMф细胞6h后用LPS(10μg/m l)刺激,用ELISA法及RT-PCR法测圈套ODNs对IL-10在蛋白及mRNA水平的影响。结果哑铃形圈套ODNs可降低在IL-10mRNA及蛋白水平的表达。结论靶向NF-κB的哑铃形圈套ODNs具有抗IL-10的作用。
Objective： Decoy - ODNs in dumbbell shape with the nuclear sequence similar to NF - kB cis - elements were synthetized in this study. To measure the anti -inflammatory action of decoy -ODNs targeting NF - kB. Methods： With carriers of cationic liposomes, decoy ODNs were transfected into pMqb cells from rats. Then the inhibiting effects of the decoy - ODNs of IL - 10 were analysized. Results Decoy - ODNs could decrease the expression of IL - 10 . Conclusions ： Decoy - ODNs targeting nuclear factor - kB can decress the expression of IL - 10 in pMФ ceils.
出处
《泰山医学院学报》
CAS
2006年第7期610-613,共4页
Journal of Taishan Medical College
基金
国家重点基金研究规划项目(G1999054203)
全军"十五"课题(2001-2003)
国家自然科学基金(30170367)
