摘要
目的:观察双黄升白颗粒(黄芪、黄精、女贞子、天花粉、骨碎补等)对白细胞减少症模型小鼠骨髓细胞增殖及粒单系集落刺激因子(GM-CSF)生成的影响,并探讨其作用机制。方法:Se-137放射源照射造成小鼠白细胞减少症动物模型,采用MTT比色法测定小鼠骨髓细胞增殖程度:采用双抗体夹心Elisa法测定小鼠血清中GM-CSF含量;采用SP免疫细胞化学方法测定培养的小鼠脾细胞胞浆中GM-CSF蛋白表达。结果:双黄升白颗粒能够促进模型小鼠骨髓细胞增殖,并能升高模型小鼠血清GM-CSF含量,促进小鼠脾细胞GM-CSF蛋白表达。结论:双黄升白颗粒能够促进白细胞减少症模型小鼠骨髓细胞增殖,升高其血清GM-CSF含量,并促进小鼠脾细胞GM-CSF蛋白表达,这可能是其具有升高白细胞作用的内在机制之一。
AIM ： To observe the effect of Shuanghuang Shengbai Granule （Radix astragli, Rhizoma polygonati, Fructus ligustri lucidi, Radix trichosanthis , Rhizoma drynariae , etc） （SHSB） on the proliferation of bone marrow cells and the growth of granulocyte-macrophage colony stimulating factor（GM-CSF） in leukopenia model mice, and to investigate the related mechanism. METHODS： Mouse leukopenia models were made by radiation, the proliferation of bone marrow ceils was determined by MTT chromatoptometry, the content of GM-CSF in serum was measured by double antibody sandwich ELISA, and the protein expression of GM-CSF in cultured spleen cells was measured by SP immunocytochemistry. RESULTS ： SHSB could promote the proliferation of bone marrow cells ; increase the content of GM-CSF in serum and enhance the protein expression of GM-CSF in spleen ceils. CONCLUSION. SHSB Granule has the improvement in enhancing the proliferation of bone marrow ceils in leucopenia model mice, increasing the content of GM-CSF in serum and the protein expression of GM-CSF in spleen cells.
出处
《中成药》
CAS
CSCD
北大核心
2007年第4期501-504,共4页
Chinese Traditional Patent Medicine
基金
上海市科学技术委员会资助项目(02DZ19136)
