摘要
目的探讨绞股蓝总皂甙(GP)对缺血再灌注损伤心肌的保护机制。方法16只大鼠随机分为4组:假手术组(Sham组),缺血再灌注组(I/R组),绞股蓝组(GP组),丹参对照组(SM组)。采用链酶亲和素-生物素-酶复合物(SABC)免疫组化检测心肌肿瘤坏死因子(TNF-α)的表达。结果I/R组TNF-α表达明显增多(P<0.01),GP组TNF-α表达较I/R组明显下降(P<0.01)。结论GP对大鼠心肌缺血再灌注损伤的保护作用与抑制TNF-α表达有关。
Objective To investigate the effects of Gynostemma Pentaphyllam（GP） on myocardial ischemia-reperfusion injury in rats （MIRI）. Methods The MIRI model was establshed by left descending anteri- or coronary artery ligation for 30min ,followed by 60min of reperfusion. 16male wistar rats were divided into four groups; sham operated control group （ Sham ）, ischemia-reperfusion group （ IR ）, GP group （ GP ）, and salvia miltiorrhizae group （ SM ）. Results Compared with Sham group, the expression of tumor necrosis factor-alph was sig- nificantly increased in IR group（ P 〈0.01 ） ,and markedly decreased in GR group （ P 〈0.01 ）. Conclusion GP has protective effects on myocardial ischemia-reperfusion injury in rats, it may be related to inhibiting the expression of tumor necrosis-alph.
出处
《咸宁学院学报（医学版）》
2007年第1期7-8,共2页
Journal of Xianning Univarsity(medical Sciences)
关键词
绞股蓝总皂甙
心肌缺血再灌注
肿瘤坏死因子
Gynostemma pentaphyllam
Myocardial ischemia-reperfusion
Tumor necrosis factor-α.
