摘要
目的研究颗粒溶素(granulysin,GNLY)、颗粒酶 B(granzyme B,GrB)及穿孔素(perforin,PF)三种免疫效应分子在原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)外周血中的表达并探讨其与 PBC 的关系。方法以正常健康人和肝炎后肝硬化患者为对照,用实时荧光定量 RT-PCR 方法检测 PBC 患者 PBMC 中 GNLY、GrB 及 PF mRNA 的含量。用 ELISA 方法检测 PBC 患者血清中 GNLY 表达情况并分析其与肝功能的相关性。结果 PBC 组 GNLY、GrB mRNA 平均拷贝数显著高于健康对照组(P<0.01)和疾病对照肝炎后肝硬化组(P<0.001),而 PF mRNA 与对照组相比差异无统计学意义。PBC 患者血清中的 GNLY 蛋白表达显著高于健康对照组(P<0.01)和乙型肝炎后肝硬化组(P<0.01)。血清中 GNLY 浓度与血清 GGT、ALP 的浓度呈正相关(P<0.01)。结论 PBC 患者 GNLY、GrB mRNA 和含量显著高于对照组,同时 ELISA 方法检测出 PBC 患者血清中 GNLY 蛋白含量显著高于对照组,GNLY、GrB 表达与 PBC 的发病存在一定的关联性,为临床 PBC 病情的监控提供可靠依据。
Objective To explore the expression of 3 immune effector molecules： granulysin （GNLY）, granzyme B（GrB）, perforin（PF） in peripheral blood of patients with primary biliary cirrhosis （PBC） and its relationship with PBC. Methods The real time quantitative reverse transcription pelymerase chain reaction（RT-PCR） was used to detect the expression of GNLY, GrB, and PF mRNA in the peripheral blood of 60 PBC patients, 60 healthy controls and 60 patients with hepatitis-related liver cirrhosis; The enzyme-linked immunospecific assay （ELISA） were used to determine the expression of GNLY protein;and the expression difference were compared between patients with different stage of PBC ;The correlation between the GNLY protein with the hepatic functions of PBC was analyzed statiscally. Results The mean GNLY mRNA copy number in PBC group was significantly higher than that in controls （ P 〈 0. 01 ） and hepatitis B related liver cirrhosis group （P 〈 0. 001 ）. Meanwhile, the serum level of GNLY in PBC was significantly higher than that in healthy controls （ P 〈 0. 01 ） and hepatitis B related liver cirrhosis group （ P 〈 0. 01 ）. The levels of serum GNLY in patients with PBC were also positively correlated with the levels of GGT, ALP. Conclusions Our results indicate that the GNLY, GrB gene xpression levels are both increased in patients with PBC . The serum level of GNLY expression is associated with progression of PBC, which is helpful to monitor the pathogenetic condition of PBC patients.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2007年第4期395-398,共4页
Chinese Journal of Laboratory Medicine
基金
国家高技术研究发展计划(2006AA02Z496)
国家自然科学基金(30471616)
上海市重大科技攻关基金(04DZ19116)
上海市重点科研支撑条件项目(051409012)
上海市青年科技启明星计划(QMX01423)
