摘要
目的:检测破骨细胞分化因子(RANKL)及其配体核因子-κB受体活化因子(RANK)及假性受体骨保护素(OPG)在胆脂瘤型中耳炎组织中的表达水平,探讨RANKL在胆脂瘤型中耳炎骨质破坏机制中的作用。方法:应用免疫组织化学SP法检测RANKL、RANK和OPG在30例中耳胆脂瘤组织和20例正常外耳道皮肤中的表达。结果:RANKL、RANK在胆脂瘤组织中表达增高,阳性细胞主要位于胆脂瘤周围组织,与正常对照组比较差异有统计学意义(t=7.758、6.482,均P<0.05);OPG的表达无明显变化,与正常对照组比较差异无统计学意义。OPG/RANKL在胆脂瘤组织中较正常对照组明显降低(t=8.183,P<0.05)。结论:RANKL及RANK在中耳胆脂瘤周围组织中表达增高,与骨质破坏密切相关。
Objective：To investigate the expressions of nuclear factor-kappa B ligand （RANKL）, receptor activator of nuclear factor-kappa B（RANK）and osteoprotegerin （OPG） and the relation of RANKL with bone- erosion in human cholesteatoma tissue. Method： Thirty cholesteatoma and twenty normal auditory canal skin specimens were investigated. The expressions of RANKL, RANK and OPG were examined by immunohistochemistry. Resuit：The overexpressions of the cytokine RANKL and RANK were found in infiltrated lymphocytes in the cholesteatoma tissue comparing with normal external meatal skin（ t = 7. 758,6. 482, P 〈0.05）; While, the expression of OPG was significantly higher in cholesteatoma tissue comparing with normal external meatal skin. the OPG/ RANKL was decreased in cholesteatoma tissue comparing with normal external meatal skin（ t = 8. 183, P 0.05）. Conclusion：This study revealed that the expressions level of RANKL and RANK were markedly increased in the perimatrix of cholesteatoma, which is closely related to the bone- erosion induced by cholesteatoma.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2007年第7期315-317,共3页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
胆脂瘤
中耳
破骨细胞分化因子
核因子-κB受体活化因子
骨保护素
Cholesteatoma ,middle ear
Nuclear factor-kappa B ligand
Receptor activator of nuclear factor- kappa B
Osteoprotegerin
