摘要
目的:研究波纹巴非蛤体液和肌肉凝集素对细胞的凝集性能及其理化性质,为开发新的凝集素来源提供基础材料。方法:利用10种动物及人的4种红细胞1、5种藻类细胞测定波纹巴非蛤的体液和肌肉凝集素的凝集活力,同时进行热稳定性、pH敏感性、糖抑制及金属离子影响试验。结果:体液和肌肉凝集素分别对家鸽、家兔血红细胞最为敏感,凝集效价最高,均为25。对藻类的凝集则具有某些专一性。体液凝集素有较高的温度耐受性,90℃处理10min仍能凝集兔血红细胞。肌肉凝集素对碱性环境较敏感,当pH≥8.0时,凝集活力消失。体液及肌肉凝集素在糖抑制及金属离子影响试验中也存在着差异。结论:波纹巴非蛤的体液与肌肉提取液均含凝集素,但所含凝集素类型不同。
Objective： Agglutinating activities and characteristics of lectins
extracted from humor and muscle of P.undufata were studied for the new
lectins source.Methods：Humor and muscular extracts from P.undufata
were detected for the agglutination with 10 kinds of erythrocytes of
animals,4 human blood types and 15 kinds of alga cells.Meanwhile
sugar-inhibiting action,thermostability and influence of pH and metal
hydronium on the activities of the two kinds of samples were
tested.Results： Pigeon and rabbit red blood cell were respectively most sensitive to the humor and muscular lectins,the agglutinating activity is 2^5 . Some alga cells were specifically effected by the two lectins. The humor agglutinins have a high thennostability that has activity after exposing to 90℃ for 10 rain. The muscular agglutinins showed a higher sensitivity to alkali canditions. There is no agglutinating activity was observed above pH 8. The two lectlns showed difference on the suger- inhibiting action and the influenee of mental hydronium. Conclusion：both humor and muscular extract from P. undufata contain leetin, and they are different in nature.
出处
《生物技术》
CAS
CSCD
2007年第1期26-29,共4页
Biotechnology
基金
福建省科技厅项目资助项目("2种入侵贝类化学防治对环境的影响及抗菌活性物质研究"
编号:2005K018)
关键词
波纹巴非蛤
凝集素
凝集活力
理化性质
Paphia undufata
Lectln
agglutinating activity
characteristics
