摘要
以葫芦科1年生攀缓植物丝瓜幼苗为材料,研究了它在低浓度不同种类盐中的吸水规律和在不同浓度的KCl溶液中以总吸水倍率和叶子中脯氨酸质量分数以及培养外液渗透压变化作为测定指标,从渗透压作为丝瓜幼苗时期吸水动力的角度,探讨其吸水动力学变化及生长规律.结果表明:低浓度(0.0010mol/L)各种盐对丝瓜幼苗的总吸水倍率的影响为LiCl〉KCl〉二次蒸馏水〉NaCl;不同浓度(0~0.0140mol/L)KCl影响下丝瓜幼苗总吸水倍率、脯氨酸质量分数、培养液进入植物体内的粒子数随KCl浓度成峰形变化,并且三者的变化基本吻合.KCl浓度为0.0010mol/L时脯氨酸质量分数达最低值,0.0040mol/L时脯氨酸的质量分数达最高值,在无盐和高盐环境下脯氨酸的大量积累是植物细胞的适应性反应,其质量分数的高低不宜作为丝瓜的抗盐性指标.脯氨酸质量分数与进入植物体内粒子数变化正相关,并拟合出关系式W=k0+ke^R0^n,W为脯氨酸的质量分数,n为进入植物体内的粒子数,k0,k,R0均为常数.本研究为探讨植物体中脯氨酸积累的生理作用及意义提供参考.
Changes in water absorbing of seedlings of cucurbitaceous towel gourd in response to changes in composition and concentration of external solution were studied. We assume the osmotic pressure as the motivity of water uptake of seedlings and take the absorption power, proline content and osmotic pressure of external solution as parameters. The hydrating effect of solutes applied at low concentrations (0. 001 0 mol/L) and different composition increased in the following sequence: sodium chloride 〈 distilled water〈 potassium chloride 〈 lithium chloride. Changes in the absorption power, leaf proline content and osmotic pressure of external solution of seedlings cultivated in potassium chloride solution applied at low and moderate concentrations (0-0. 014 0 mmol/L) in response to changes in concentration of external solution were basically tally. All had a peak value. At salinity of 0. 001 0 and 0. 004 0 mol/L, proline accumulation respectively reaches its lowest and highest point , which shows proline content cannot act as an index to reflect the stress tolerance of towel gourd. Proline accumulation under high salinity and 0 salinity results from the disorder of cell structure and function. The number of particles coming into the seedlings was significant positively correlated with the leaf proline content. It offered us reference for studying the physiology significance of proline accumulation. The above equation reduce to the type relation as follows: w = k0 + ke^R0^n , where w was the leaf proline content and n was the number of particles coming into the seedlings and k0, k, R0 were constant.
出处
《河北大学学报(自然科学版)》
CAS
北大核心
2007年第2期160-165,共6页
Journal of Hebei University(Natural Science Edition)
基金
河北省自然科学基金资助项目(200600962)
关键词
吸水倍率
渗透压
脯氨酸
渗透调节
absorption power
osmotic pressure
proline
osmotic adjustment