摘要
首次对金钱松内生真菌进行了分离,采用改良氯化三苯四氮唑显色(TTC)微量稀释法,对其活性菌株进行筛选;以11种常见导致食品腐败的细菌、酵母菌、霉菌为指示菌,研究了活性菌株的抗菌能力。结果表明:金钱松内存在着广泛的具有抗菌活性的内生真菌,占内生真菌总数的28．5%,高活性菌株占15．2%,其中JJ18菌株发酵粗提物具有强烈的抗细菌、酵母菌和丝状真菌的广谱抗菌能力,JJ314抗细菌、酵母菌活性强。金钱松内生真菌抗菌活性是潜在的食品防腐抗菌资源。筛选方法准确可靠。
Endophytes fungus strains were screened firstly from Pseudolarix kaempferi Gord., and the active strains were screened with improved TTC（3.5-triphenyltetrazolium chloride） microdilution method. MIC（minimum inhibitory concentration） and MBC（minimum bactericidal concentration） were detected against eleven food spoilage strains. The results showed that 28.5% endophytes strains have antimicrobial activity where 15.2% endophytes have higher antimicrobial activity among which JJ 18 strains have broad-spectrum antimicrobial activity to bacterium, yeast and mycelial fungus. While JJ314 has strong antimicrobial activity to bacterium and yeast. Antimicrobial active endophytes strains from Pseudolarix kaempferi Gord. are potential resources for foods antiseptic. Microdilution method is accurate and reliable.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第4期199-203,共5页
Food Science
基金
江苏省2004年度博士研究生创新计划项目(xm04-57)
关键词
金钱松:内生真菌
抗菌活性
Pseudolarix kaempferi Gord.
endophyte fungus
antimicrobial activity
