摘要
从2-酮基-D-葡萄糖酸产生菌荧光假单胞菌A46异常发酵液中分离得到1种噬菌体,将其命名为KS461-2。电镜观察表明,噬菌体KS461-2呈蝌蚪状。根据ICTV对病毒的分类标准,噬菌体KS461-2属长尾噬菌体科,为烈性噬菌体。噬菌体KS461-2对宿主菌的最佳感染复数为0．01,温度和pH对其稳定性具有显著的影响。一步生长曲线显示,噬菌体KS461-2的潜伏期约30min,裂解期约135min,裂解量为24。SDS-PAGE显示,噬菌体KS461-2有5种主要的结构蛋白,其分子量分别为66．0、61．0、42．5、34．7和14．4kDa。
A phage, named KS461-2, was separated from the abnormal fermentation liquid of Pseudomonas fluorescens A46 strain which produced 2-keto-D-gluconic acid. The KS461-2 was tadpole-like bacteriophage as observed by electron microscope. According to ICTV（Intemational Committee for Taxonomy of Viruses）, it belongs to the Siphoviridae family and is a virulent phage. The optimal MOI（multiplicity of infection） of KS461-2 is 0.01. Temperature and pH value had remarkable effects on the stability of phage KS461-2. One-step growth curve showed that the latent period is 30min, the burst period 135 min, and the average burst size 24 phage particles/infected cell. SDS-PAGE profiles indicated that KS461-2 contains five structural proteins, and their molecular masses are 66.0, 61.0, 42.5, 34.7 and 14.4kDa respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第4期187-191,共5页
Food Science
基金
国家高技术产业化示范工程项目(200306)
