摘要
目的研究自发免疫耐受大鼠移植肝内转录因子Foxp3的变化。方法双袖套法建立大鼠原位肝脏移植模型。取肝组织,通过实时荧光定量PCR检测大鼠移植肝内Foxp3 mRNA的表达;Western blot检测肝内Scurfin蛋白含量的变化。结果自发耐受组大鼠肝移植1周内Foxp3 mRNA及其蛋白产物Scurfin的表达明显下降,在7d左右开始上升,14d达到峰值,30d后Foxp3 mRNA的表达开始下降,但仍高于正常值的(2.86±0.12)×10-3(P<0.05)。结论转录因子Foxp3可能参与大鼠肝移植自发免疫耐受的形成。
Objective To investigate the expression of transcription factor Foxp3 in the orthotopic liver transplantation by using the inbred rats with spontaneous immune tolerance. Methods The model of orthotopic liver transplantation was established on inbred rats according to double-sleeve technique. The total RNA that was isolated from liver was reversely transcribed into cDNA. The method of real-time fluorescence quantitative PCR （RFQ-PCR） was used to analyze the expression level of Foxp3 mRNA in tolerance group and syngeneic group, respectively. The expression of Scurfin in hepatic tissue was assayed by Western blot and then was analyzed by computer imaging system. Results The expression levels of Foxp3 mRNA and Scurfin in the transplanted liver were significantly lower than those of normal liver within the first week after transplantation. The level of Foxp3 mRNA began to increase on day 7 and reached the peak point on day 14. The expression level of Foxp3 mRNA began to decrease on day 30 but was still higher than the normal value （P〈0.05）. The Western blot showed resemble changes on that of Scurfin. Conclusion Transcription factor Foxp3 may play an important role in the spontaneous immune tolerance in the orthotopic liver transplantation of inbred rat.
出处
《中国普外基础与临床杂志》
CAS
2007年第2期138-141,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
江苏省医学"135"重点科室资助项目(编号:135-43)~~
关键词
FOXP3
原位肝移植
免疫耐受
Foxp3 Orthotopic liver transplantation Immune tolerance
