摘要
试验以辽宁绒山羊和内蒙古绒山羊为实验动物,采用PCR-RFLP技术对κ-酪蛋白基因(CSN3)的TaqⅠ酶切多态性进行分析,结果表明:辽宁绒山羊和内蒙古绒山羊2个山羊群体中均存在CSN3基因TaqⅠ酶切位点的多态性,且均有A和B 2个等位基因。辽宁绒山羊群体等位基因A和B的基因频率分别为0.46和0.54;内蒙古绒山羊群体中等位基因A和B的基因频率分别为0.31和0.69。CSN3基因TaqⅠ酶切位点的基因型分布在辽宁绒山羊群体中极显著(P<0.01)偏离Hardy-Weinberg平衡定理,在内蒙古绒山羊群体中符合Hardy-Weinberg平衡定理(P>0.05),但在辽宁绒山羊与内蒙古绒山羊2个群体间存在极显著(P<0.01)差异。
Using the technique of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）, genetic polymorphism of Taq Ⅰ site of κ-casein （CSN3） gene in Liaoning cashmere goats and Inner Mongolian cashmere goats were tested. The results showed that the Taq Ⅰ sites controlled by allele A and B were polymorphic in Liaoning cashmere goats and Inner Mongolian cashmere goats. The allele frequencies of A and B were 0.46 and 0.54 in Liaoning cashmere goats, respectively; and the allele frequencies of A and B were 0. 31 and 0. 69 in Inner Mongolian cashmere goats, respectively. The genotype distribution of Taq Ⅰ sites in Liaoning cashmere goats didn＇t fit significantly Hardy-Weinberg law （P 〈 0.01）, while the Taq Ⅰ sites in Inner Mongolian cashmere goats fitted Hardy-Weinberg law （P 〉 0.05）. The difference of the genotype distribution between Liaoning cashmere goats and Inner Mongolian cashmaere goats was very significant（P 〈 0.01）.
出处
《中国畜牧杂志》
CAS
北大核心
2007年第7期4-6,共3页
Chinese Journal of Animal Science
