摘要
目的研究高氧肺损伤早产鼠肺组织硫氧还蛋白(Trx)的表达及其临床意义。方法早产新生SD大鼠128只,生后d1随机分为空气组和高氧组,每组64只。分别于d1、4、7、10、14提取肺组织总RNA,采取半定量逆转录聚合酶链反应(RT-PCR)测定Trx mRNA水平;免疫组织化学方法检测肺组织切片凋亡蛋白半胱氨酸蛋白酶-3(Caspase-3);HE染色观察肺组织病理变化。结果高氧组肺发育明显受阻,与空气组比较,高氧暴露后Trx mRNA表达显著增强,且于d10时达高峰(P<0.05,0.01)。Caspase-3表达显著升高(P<0.05,0.01)。结论高氧上调早产鼠肺组织Trx mRNA的表达;肺组织Trx mRNA的表达增高可能在早产鼠高氧肺损伤的发生发展中发挥重要保护作用。
Objective To investigate the changes and potential role of the expression of thioredoxin （Trx） in lung of premature rats exposed to hyperoxia and its clinical significance. Methods In the first day after delivery,128 preterm SD rats were randomly divided into air group （n =64） and hyperoxia group（n =64）. The rats were respectively killed in d1,4,7,10,14. Total RNA of lung was isolated and the expression ofTrx mRNA was detected by reverse transcription polymerase chain reaction（ RT - PCR）. Immunohistochemistry was used on lung sections to detect the expression of Caspase - 3. The sections of lung were stained with HE in order to assess lung histologic changes. Results Rats in hyperoxia group showed typical lung injury, which was characterized by delay of lung development. Compared with air group, Trx mRNA expression in hyperoxia group markedly increased, peaking at d10 （P 〈0.05,0.01 ）. Expression of Caspase -3 increased in hyperoxia group as well（ P 〈 0.05,0.01 ）. Conclusion Trx mRNA expression increasing can be induced by hyperoxia, which might play importantly protective role in development of hyperoxia - induced lung injury.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第6期410-411,共2页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金项目资助(30672261)
