摘要
目的：建立HPLC法测定注射用黄藤素粉针剂中盐酸巴马汀的含量.方法：用十八烷基硅烷键合硅胶为填充剂,乙腈-水-磷酸二氢钾-十二烷基磺酸钠（300 ml∶200 ml∶1.7 g∶0.85 g）为流动相,检测波长为345 nm.结果：盐酸巴马汀在0.0101～0.0504 μg/ml范围内线性关系良好,相关系数为0.9997 （n = 5 ）.平均回收率为99.35% （RSD = 0.99%, n = 5）.结论： 该法简便、快速、准确、稳定,重现性好.
Objective： To establish a method for determination of the content of palmatine hydroehloride in lyophilized fibrauretinum for injection by HPLG. Method：The column of C18 was used and the mobile phase consisted of a mixture of aeetonitrile - water - potassium dihydrogen phosphate - sodium dodecyl sulfonate （300 ml ： 200 ml ： 1.7 g ： 0. 85 g）. The detection wavelength was set at 345 nm. Re,lilt. palmatine hydrochloride showed a good linear relationship in the range of 0. 0101-0. 0504μg/ml （ r =0. 9997, n = 5）. The average recovery was 99. 35% （ RSD = 0.99%, n=5）. Conclusion：The HPLC method for determination of the content of palmatine hydrochloride in lyophilized fibranretinum is simple, rapid, accurate and steady with good repeatability.
出处
《西南国防医药》
CAS
2007年第2期191-193,共3页
Medical Journal of National Defending Forces in Southwest China
关键词
黄藤素粉针剂
盐酸巴马汀
HPLC
fibrauretinum injection
palmatine hydrochloride
FIPLC
