摘要
目的:进一步观察谷氨酰胺(Glutamine,Gln)对过度训练大鼠肠粘膜屏障功能的保护作用并探讨其可能的作用机制。方法:120只健康雄性SD大鼠随机分为普通训练组(C组)(n=30)(作为对照组)、补充Gln+普通训练组(CG组)(n=30)、过度训练组(O组)(n=30)和补充Gln+过度训练组(OG组)(n=30)。采用模拟过度训练大鼠模型,应用形态学手段结合免疫组化和放射免疫技术,观测肠道形态、固有层IgA浆细胞、粘液IgA、Ⅱ型磷脂酶A2(Ⅱ型PLA2)和溶菌酶分泌等指标的变化情况。结果:Gln能明显减轻过度训练大鼠肠粘膜结构损伤。OG组IgA浆细胞计数及其平均吸光度、粘液IgA含量、Ⅱ型PLA2水平和溶菌酶含量与O组比较,显著增加(P<0.01)。结论:Gln可能通过刺激大鼠小肠肠壁分泌Ⅱ型PLA2和溶菌酶的作用来维护过度训练大鼠肠粘膜屏障功能。
Objective ： To investigate the protective effects of glutamine on intestinal mucosal barrier in overtraining rat model and explore its possible mechanisms. Methods ：120 healthy male SD rats were randomly divided into the control group （group C） （ n = 30）, Gln＋ control group （group CG） （ n = 30）, overtraining group（group O） （ n = 30）, Gln ＋ overtraining group（group OG） （ n = 30）. Adopted overtraining rat model, the histological appearance of intestine, mucosal IgA immunity and its mechanisms, type Ⅱ phospholipase A2（type Ⅱ PLA2）, lysozyme were measured by means of the electron microscope technique, radioimmunoassay,immunohistochemical and image analysis and other corresponding methods respectively. Results： Glutamine could significantly attenuate intestinal mucosal injuries and ameliorate bacteria translocation, the number of IgA-containing cells and their mean optical density（A） and the level of mucus IgA and the type Ⅱ PLA2 activity and lysozyme content of intestinal mucosa were all significantly decreased in group OG compare with group O. Conclusion：The possible mechanisms might involve the Gln can significant stimulate the small intestine of mice to excrete type Ⅱ PLA2 and lysozyme and resulting in the increase of type Ⅱ PLA2 and lysozyme contents in the intestinal tract and enhancing the function of mucosal barrier of intestine.
出处
《武汉体育学院学报》
CSSCI
北大核心
2007年第4期67-70,83,共5页
Journal of Wuhan Sports University
基金
聊城大学自然科学基金重点资助项目(XJ200301)
关键词
过度训练
肠粘膜屏障
Ⅱ型PLA2
溶菌酶
大鼠
运动生物化学
overtraining
intestinal mucosal barrier
type Ⅱ phospholipase A2
lysozyme
mouse
sport biochemistry
