摘要
目的 探索肾母细胞瘤患儿预后监测的新方法。方法 应用表面增强激光解析电离飞行时间质谱（SELDI—TOF—MS）技术检测肾母细胞瘤患儿术前后血清蛋白质标记物的表达差异。肾母细胞瘤患儿血清标本100例，其中术前30例、术后2周24例、术后3个月和6个月各23例，正常儿童血清标本20例，以生物信息学方法支持向量机分析质谱数据。结果 肾母细胞瘤患儿手术（包括根治术和姑息性切除术）前后血清蛋白质标记物有明显差异。筛选出2个质荷比（m／z）位于6984．0、6455．0处的蛋白质标记物：术前30例标本2种蛋白质标记物表达强度分别为299．8±134．6、1037．0±375．9，与正常对照组（表达强度分别为1110．6±213．8、2268．7±1058．0）比较差异有统计学意义（P〈0．01）；19例根治术患儿术后2周2种蛋白质标记物表达强度分别为1101．2±221．8、2065．2±660．5，与术前组比较差异有统计学意义（P〈0．01），与正常对照组比较差异无统计学意义（P〉0．05）。2例根治术和3例姑息性切除患儿术后2周2种蛋白质标记物表达强度分别为358．1±213．3、1307．9±402．2，与术前组比较差异无统计学意义（P〉0．05）。术后3、6个月复查发现，持续高表达者均无复发转移征象，持续低表达者为瘤体未切净或已有远处潜在转移者。结论 血清蛋白质标记物检测为肾母细胞瘤术后患者预后判断提供了一种可能的新方法。
Objective To explore the new method to monitor the prognosis of nephroblastoma. Methods We applied the surface-enhanced laser desorption/ionization time of flight-mass spectrometry （SELDI-TOF-MS） to detect the differences in biomarkers between preoperative and postoperative serums of nephroblastoma. 100 serum samples from nephroblastoma： 30 preoperative nephroblastomas, 24 postoperative nephroblastomas（2 weeks after operation）, 23 postoperative nephroblastoma （3 months and 6 months after operation respectively） , 20 serum samples from healthy children. The data of spectra were analyzed by support vector machine（SVM）. Results There was significant difference in the serum biomarkers between preoperative and postoperative patients with nephroblastoma. Two peaks with m/z of 6984.0, 6455.0 were acquired. Compared with normal children＇s group（the intensity of two biomarks：1110. 6±213. 8,2268. 7±1058. 0） , the expression of two biomarks with preoperative nephroblastoma patients（ the intensity of two biomarks： 299.8 ±134.6,1037.0 ± 375.9 ）was lower（P〈0.01）. Compared with the preoperative group, the expression of 19 postoperative patients （the intensity of two biomarks： 1101.2 ± 221.8,2065.2 ± 660.5 ）with radical correction was higher （P〈0.01）; while compared with normal children＇s group, the expression was slightly lower but the difference did not reach statistic significance （P〉0.05）. Compared with the preoperative group , the expression of 2 other eases with radical correction and 3 eases of condoning excision （the intensity of two biomarks： 504.4± 86.1,1171.54±233. 8）were slightly higher but the difference did not reach sta- tistic significance （P〉0.05）. By followup in 3 months and 6 months, the patients with constant high level had no recurrence or metastasis, while the patients with low expression didn＇t got radical cure or had existed potential metastasis. Conclusions The differences in biomarkers between preoperative and postoperative
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2007年第4期224-227,共4页
Chinese Journal of Urology
基金
国家自然科学基金资助项目（30571930）
关键词
肾母细胞瘤
生物学标记
预后
Nephroblastoma
Biological markers
Prognosis
