摘要
测定骨髓转移神经母细胞瘤（ＮＢ）Ｎ－ｍｙｃ扩增情况及评价其与预后的关系。采用差示ＰＣＲ法检测１７例骨髓转移ＮＢ的Ｎ－ｍｙｃ拷贝数，并随访１年。结果，１２例（７０．５９％）证实有Ｎ－ｍｙｃ扩增。Ｎ－ｍｙｃ扩增与ＮＢ分期及患者预后均成显著相关（分别为Ｐ＜０．０５，Ｐ＜０．０１）。有Ｎ－ｍｙｃ扩增者预后差，且扩增倍数越高则预后越差。因此，Ｎ－ｍｙｃ扩增对于ＮＢ分期及预后评估均为有价值的指标，它可能与ｃ－ｍｙｃ协同调控ＮＢ的生物学特性。差示ＰＣＲ作为一种建立在内参照原理上的新方法可定量测定基因拷贝数，比Ｓｏｕｔｈｅｒｎ杂交更简便、快速、灵敏、精确，适于临床应用。尤值一提的是，该法需样品量很少，且无同位素沾染。
The differential polymerase chain reaction(D-PCR)of 17 cases with myelometastasized Neuroblastoma was measured to determine the numbers of N-myc copy amplification and followed-up for one year.N-myc amplification was confirmed in 12 patients(70.6%)and was correlated with NB staging and patient's outcome(P<0.05 and P<0.01 respectively>.The prognosis of patients with N-myc amplification was poor.The authors demonstrate N-myc amplification is a valuable factor of evaluating the staging and prognosis of NB,it may cooperate with c-mye gene to control the biological characteristics of NB,D-PCR is a new method based on intracontrast principle to determine gene copy quantitatively.Compared with Southern hybridization,it is simple,fast,sensitive,accurate and suitable to clinical use.Furthermore,it needs only a small amount of sample without isotopes contamination.
出处
《中华小儿外科杂志》
CSCD
1996年第4期195-198,F004,共4页
Chinese Journal of Pediatric Surgery
基金
国家自然科学基金
关键词
神经母细胞瘤
癌基因
基因扩增
聚合酶链反应
Neuroblastoma
Oncogene
Gene amplification
Polymerase chain reaction.
